department of pharmacology

Taylor Laboratory

mRNA cleavage and polyadenylation




In collaboration with Yongsheng Shi (UC Irvine) we are investigating the structure and function of the human pre-mRNA 3’ processing complex. The 3’ processing complex is responsible for proper transcription of nearly every gene in the eukaryotic cell, as the ends of almost all transcripts (excluding histone transcripts in some organisms) are generated by endonucleolytic cleavage and polyadenylation; processes orchestrated by the 3’ processing complex. Based largely on in vitro experiments, the pre-mRNA 3’ processing complex is believed to consist of approximately 15 different polypeptides. One goal regarding this project is to obtain an initial three-dimensional reconstruction of the 3’ processing complex, as well as that of a sub-complex called cleavage and polyadenylation specific factor (CPSF). A second goal is to further characterize the protein-protein interactions of the various subunits that comprise the pre-mRNA 3’ processing complex.