Janos Kerner, Ph.D.
Phone: (216) 368-5358
Janos Kerner graduated from Humboldt University in Berlin (Germany) where he received
his M.S. in Biology/Plant Biochemistry. He accepted a teaching/research position
in the Department of Biochemistry at Medical University of Pecs (Hungary) where
he received his Ph.D. in biochemistry. He held a faculty position at Medical University
at Pecs until 1994. During his faculty appointment in the Department of Biochemistry
at the Medical University of Pecs, he worked for several years as a visiting faculty
in the Department of Biochemistry at Michigan State University. He joined the faculty
in the Department of Nutrition at CWRU in 1995.
The elucidation of the functions of carnitine acyltransferases and acylcarnitines
in intermediary metabolism and the investigation of the regulation of mitochondrial
malonyl-CoA sensitive carnitine palmitoyltransferase (CPT-I).
My past research focused in method and assay developments for identification and
quantitation of individual acylcarnitines. Since then I have shown that carnitine
is involved in specific detoxification reactions, and in modulating the acyl-CoA/CoASH
ratio in cellular compartments.
My current research is in the area of mitochondrial fatty acid oxidation with special
emphasis on the malonyl-CoA sensitive carnitine palmitoyltransferase. CPT-I exists
in two isoforms, the liver and muscle specific isoform, and catalyzes the rate controlling
step of mitochondrial fatty acid oxidation.
Consequently, its regulation (allostericaly by malonyl-CoA and covalently by phosphorylation)
is central to several pathological conditions. Our aim is to elucidate how the two
isoforms are regulated in vivo under physiological and pathological conditions using
a multifaceted approach which involves enzymological and immunological methods as
well as proteomics.