department of pharmacology

Derek Taylor, Ph.D.

Derek Taylor

Assistant Professor

Phone: (216) 368-0684
Fax: 216-368-1300
E-mail: derek.taylor@case.edu
W333

Mt. Sinai Scholar
B.S.: Fort Lewis College, Durango CO
Ph.D.: University of California, San Diego

Laboratory

Research Interest

Regulation – and deregulation – of gene expression are critical events for every process within the cell. Alteration of these intricate processes, for example as consequences of genetic defects or bacterial/viral infections, can readily lead to one of many human ailments. The control of these processes is commonly modulated by multi-protein complexes; in fact, proteins rarely act alone, but interact intimately and precisely with other proteins and nucleic acids to properly perform their cellular functions. My laboratory studies the structure and molecular mechanisms of macromolecular machines involved in DNA maintenance and RNA maturation and biogenesis. We use cryo-electron microscopy and single particle reconstruction techniques as a primary tool for visualizing these complexes and, thus, to better understand their functions.

Selected publications

Corriveau, M., Mullins, M.R., Baus, D., Harris, M.E., & Taylor, D.J. (2013) Coordinated Interactions of Multiple POT1-TPP1 Proteins with Telomere DNA. J. Biol. Chem. In Press.

Tsybovsky, Y., Orban, T., Molday, R.S., Taylor, D., & Palczewski, K. (2013) Molecular organization and ATP-induced conformational changes of ABCA4, the photoreceptor-specific ABC transporter. Structure, 21:854-860.

Lobo, G.P., Amengual, J., Baus, D., Shivdasani, R.A., Taylor, D., & von Lintig, J. (2013) Genetics and diet regulate vitamin A production via the homeobox transcription factor ISX. J. Biol. Chem., 288:9017-9027.

Taylor, D.*, Unbehaun, A., Li, W., Das, W., Lei, S., Lao, H., Grassucci, R.A., Pestova, T.V., & Frank, J. (2012) Cryo-EM structure of the mammalian eRF1- eRF3-associated termination complex. Proc Natl Acad Sci U S A, 109:18413-8.

Krokowski, D., Gaccioli, F., Majumder, M., Mullins, M.R., Yuan, C.L., Papadopoulou, B., Merrick, W.C., Komar, A.A., Taylor, D., & Hatzaglou, M. (2011) Characterization of hibernating ribosomes in mammalian cells. Cell Cycle. 10(16):1-12.

Taylor, D.J., Podell, E.R., Taatjes, D.J., & Cech, T.R. (2011) Multiple POT1-TPP1 proteins coat and compact long telomeric single-stranded DNA. J. Mol. Biol. 2011. 410:10-17.

Speir, J.A., Taylor, D.J., Natarajan, P., Pringle, F.M., Ball, L.A. & Johnson J. E. (2010) Evolution in Action: N and C Termini of Related T=4 Viruses Exchange Roles as Molecular Switches. Submitted to Structure.

Taylor, D. J., Devkota, B., Huang, A., Topf, M., Narayanan, E., Sali, A., Harvey, S., & Frank, J. (2009) Comprehensive Molecular Structure of the Eukaryotic Ribosome. Structure. 17:11591-1604

Shi, Y., Campigli Di Giammartino,D., Taylor, D., Sarkeshik, A., Rice, W.J., Yates III, J.R., Frank, J., & Manley, J.L. (2009) Molecular Architecture of the Human pre-mRNA 3’ Processing Complex. Mol. Cell. 33:365-376.

Grassucci, R. A.,Taylor, D. J., and Frank, J. (2008) Preparation of Macromolecular Complexes used in Cryo-Electron Microscopy. Nat Protoc. 3:330-339.

Grassucci, R. A.,Taylor, D. J., and Frank, J. (2007) Visualization of Macromolecular Complexes Cryo- Electron Microscopy with FEI Tecnai TEMs. Nat Protoc. 2:3239-3246.

Frank, J., Gao, H., Sengupta, J., Gao, N., andTaylor, D.J. (2007) The process of mRNA-tRNA Translocation. Proc Natl Acad Sci U S A, 104:19671-8.

Taylor, D.J., Nilsson, J., Merrill, A.R., Andersen, G.R., Nissen, P., and Frank, J. (2007) Structures of modified eEF2•80S ribosome complexes reveals the role of GTP hydrolysis in translocation. EMBO J. 26: 2421 – 2431.