department of pharmacology

Krzysztof Palczewski, Ph.D.

2014 Publications

Mustafi, D., S. Kikano, and K. Palczewski. Serial block face-scanning electron microscopy: a method to study retinal degenerative phenotypes. Current Protocols in Mouse Biology. 2014, 4, 197-204.

Palczewska G., F. Vinberg, P. Stremplewski, M. P. Bircher, D. Salomǁ, K. Komar, J. Zhang, M. Cascella, M. Wojtkowski,V. J. Kefalov, and K. Palczewski. Human infrared vision is triggered by two−photon chromophore isomerization. Proc. Natl. Acad. Sci. 2014, 111, E5445-E5454.
Supplemental 1
Supplemental 2
Supplemental 3

Yu, G., X. Wu, N. Ayat, A. Maeda, S. Gao, M. Golczak, K. Palczewski, Z-R. Lu. Multifunctional PEG retinylamine conjugate provides prolonged protection against retinal degeneration in mice. Biomolecules 2014,15, 4570-4578.

Park, S., C. Li, F. J. Haeseleer, K. Palczewski, and J. B. Ames. Structural Insights into Activation of the Retinal L-type Ca2+ Channel (Cav1.4) by Ca2+-binding Protein 4 (CaBP4). J. Biol. Chem. 2014, 289, 31262-31273.

Baker B.Y, W. Shi, B. Wang, and K. Palczewski. High Resolution Crystal Structures of the Photoreceptor Glyceraldehyde 3-Phosphate Dehydrogenase (GAPDH) with Three and Four-bound NAD Molecules. Protein Science 2014, 23, 1629-1639.
Supplemental 1
Supplemental 2

Palczewski K. The Friedenwald lecture: Chemistry and Biology of the Initial Steps in Vision. Invest. Ophthal. Visual Sci. 2014, 55,6651-6672
Introduction. Dr. Arthur S. Polans. Introducing Krzysztof Palczewski, the 2014 recipient of the Friedenwald Award. Invest. Ophthal. Visual Sci. 2014, 55,6649-6650
Supplemental 1

Sundermeier, T.R., Jin H., Kleinjan M.L., Mustafi D., Licatalosi, D.D., and Palczewski K. Argonaute HITS-CLIP Reveals a Snapshot of miRNA Gene Regulation in the Mammalian Retina. Biochemistry 2014, 53, 5831-5833.
Supplemental 1
Supplemental 2

Amengual J., N. Zhang, M. Kemerer, T. Maeda, K. Palczewski, J. von Lintig. STRA6 is critical for cellular vitamin A uptake and homeostasis. Hum. Mol. Genetics 2014, 23, 5402-5417.
Supplemental 1

Yuan S., S. Filipek, K. Palczewski, H. Vogel. Activation of G-protein-coupled receptors correlates with the formation of a continuous internal water pathway. Nat. Commun. (2014), 5:4733 doi: 10.1038/5733.
Supplemental 1

Maeda, A. and K. Palczewski. Retinal degeneration in animal models with a defective visual cycle. Drug Discovery Today: Disease Models. 2014, 10, e163-e172.

Sundermeier RT, N. Zhang, F. Vinberg, D. Mustafi, H. Kohno, M. Golczak, X. Bai, A. Maeda, V. J. Kefalov, and K. Palczewski. DICER1 is Essential for Survival of Post-Mitotic Rod Photoreceptor Cells in Mice. FASEB J. 2014, 28, 3780-3791
Supplemental 1

Palczewska G, M. Golczak, D.R. Williams, J.J. Hunter, and K. Palczewski. Endogenous fluorophores enable two-photon imaging of the primate eye. Invest. Ophthal. Visual Sci. 2014 55:4438–4447.

Chen, Y., E.R. Farquhar, M.R. Chance, K. Palczewski, and P.D. Kiser, Insights into substrate specificity and metal activation of mammalian tetrahedral aspartyl aminopeptidase. J Biol Chem, 2012. 287(16): p. 13356–70.
Supplemental 1

Palczewska G., Z. Dong, M. Golczak, J. J. Hunter, D.R. Williams, N.S. Alexander, and K. Palczewski. Noninvasive two–photon fluorescence microscopy imaging of mouse retina and RPE through the pupil of the eye. Nature Med. 201420, 785-789.
Supplemental 1
Movie 1
Movie 2

Comar W. D., S. M. Schubert, B. Jastrzebska, K. Palczewski, and A.W. Smith. Mobility and clustering of the opsin G protein-coupled receptor in live cells with time-resolved fluorescence spectroscopy. J. Am. Chem. Soc. 2014, 136, 8342-8349.

Sui, X, P. D. Kiser, T. Che, P. R. Carey, M. Golczak,, W. Shi, J. von Lintig, and K. Palczewski. Analysis of carotenoid isomerase activity in a prototypical carotenoid cleavage enzyme, apocarotenoid oxygenase (ACO). J. Biol. Chem 2014, 289, 12286-12299.

Benlian Wang, B., Y. Tsybovsky, K. Palczewski, and M. R. Chance. Reliable Determination of Site–specific in vivo Protein N–Glycosylation based on Collision–Induced MS/MS and Chromatographic Retention Time. J. Am. Soc. Mass Spect. 2014 25, p.729-741.

Maeda, A., G. Palczewska, M. Golczak, H. Kohno, Z. Dong, T. Maeda, K. Palczewski. Two-photon microscopy reveals early rod photoreceptor cell damage in light−exposed mutant mice. Proc. Natl. Acad. Sci. 2014, 111, E1428-E1437.
Supplemental 1
Supplemental 2

Tu, X. and K. Palczewski. The macular degeneration-linked C1QTNF5 (S163) mutation causes higher-order structural rearrangements. J. Struct. Biol. 2014, 186, p. 186, 86-94.
Supplemental 1

Chen Y., Jastrzebska, B., P. Cao, J. Zhang, B. Wang, W. Sun, Y. Yuan, Z. Feng, K. Palczewski. Inherent instability of the retinitis pigmentosa P23H mutant opsin. J. Biol. Chem. 2014, 289, p. 9288-9303.
Movie 1
Movie 2

Tsybovsky, Y. and K. Palczewski. Expression, purification and structural properties of ABC transporter ABCA4 and its individual domains. Protein Exp. and Purif. 2014, 97, 50-60.

Orban T, B. Jastrzebska, and K. Palczewski. Structural approaches to understanding retinal proteins needed for vision. Current Opinion in Cell Biology 2014, 27: p. 32–43.

Sundermeier, R. S., F. Vinberg, D. Mustafi, X. Bai, V. J. Kefalov, K. Palczewski. R9AP overexpression alters phototransduction kinetics in iCre75 mice. Invest. Ophthal. Visual Sci. 2014, 55, 1339-1347.

Sakami S, A.V. Kolesnikov, V. J. Kefalov, K. Palczewski. P23H opsin knock−in mice reveal a novel step in retinal rod disc morphogenesis. Human Mol. Genetics 2014, 23, 1723-1741.

Wu, X., G. Yu , C. Luo, A. Maeda, N. Zhang, D. Sun, Z. Zhou, A. Puntel, K. Palczewski, and Z-R. Rong Lu. Synthesis and evaluation of a nanoglobular dendrimer 5-aminosalicylic acid conjugate with a hydrolyzable Schiff base spacer for treating retinal degeneration. ACS Nano 2014, 8, 153-161.

Bolze CS, R. E. Helbling, R. L. Owen, A. R. Pearson, G. Pompidor, F. Dworkowski, M. R. Fuchs, J. Furrer, M. Golczak, K. Palczewski, M. Cascella, and A.Stocker, Human cellular retinaldehyde-binding protein has secondary thermal 9-cis-retinal isomerase activity. J. Am. Chem. Sci. 2014, 136, 137-146.
Supplemental 1

Kiser, P. D., M. Golczak, and K. Palczewski, Chemistry of the Retinoid (Visual) Cycle. Chem. Rev. 2013, 114, 194-232.